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e99  (Bethyl)


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    Bethyl e99
    E99, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 954 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e99/product/Bethyl
    Average 96 stars, based on 954 article reviews
    e99 - by Bioz Stars, 2026-02
    96/100 stars

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    AAE inhibited polyol pathway activity and glycogen accumulation in HFD/STZ mice. The levels of aldose reductase ( a ), fructose ( b ), methylglyoxal ( c ), and carboxymethyl-lysine ( d ) were detected by a commercially available ELISA kit. ( e ) Pathological changes were evaluated by periodic acid–Schiff (PAS) staining, showing glycogen accumulation and abnormal polysaccharides (400× magnification). ( f ) PAS-positive areas in kidney tissues were quantified. Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; * p < 0.05, ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; HFD: high-fat diet; PAS: periodic acid–Schiff; STZ: streptozotocin.

    Journal: International Journal of Molecular Sciences

    Article Title: Metabolic and Antioxidant Modulation by Artemisia indica Willd. Aqueous Extract in Glucose and Cholesterol Dysregulation

    doi: 10.3390/ijms27010297

    Figure Lengend Snippet: AAE inhibited polyol pathway activity and glycogen accumulation in HFD/STZ mice. The levels of aldose reductase ( a ), fructose ( b ), methylglyoxal ( c ), and carboxymethyl-lysine ( d ) were detected by a commercially available ELISA kit. ( e ) Pathological changes were evaluated by periodic acid–Schiff (PAS) staining, showing glycogen accumulation and abnormal polysaccharides (400× magnification). ( f ) PAS-positive areas in kidney tissues were quantified. Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; * p < 0.05, ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; HFD: high-fat diet; PAS: periodic acid–Schiff; STZ: streptozotocin.

    Article Snippet: A normalized analysis was conducted by measuring urinary albumin (Mouse Albumin AssayMax ELISA Kit, Assaypro, Saint Charles, MO, USA, Cat# EMA3201-1) and urinary creatinine (Cayman Creatinine ELISA Kit, Item No. 502330, Michigan, USA), and subsequently, the urinary albumin-to-creatinine ratio (UACR) was calculated [ ].

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Staining, Derivative Assay, Control

    AAE enhanced antioxidant defense in HFD/STZ mice. The content of antioxidant enzymes, including glutathione (GSH) ( a ), superoxide dismutase (SOD) ( b ), and catalase ( c ), was detected by ELISA assays. ( d ) Malondialdehyde (MDA). Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; HFD: high-fat diet; STZ: streptozotocin.

    Journal: International Journal of Molecular Sciences

    Article Title: Metabolic and Antioxidant Modulation by Artemisia indica Willd. Aqueous Extract in Glucose and Cholesterol Dysregulation

    doi: 10.3390/ijms27010297

    Figure Lengend Snippet: AAE enhanced antioxidant defense in HFD/STZ mice. The content of antioxidant enzymes, including glutathione (GSH) ( a ), superoxide dismutase (SOD) ( b ), and catalase ( c ), was detected by ELISA assays. ( d ) Malondialdehyde (MDA). Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; HFD: high-fat diet; STZ: streptozotocin.

    Article Snippet: A normalized analysis was conducted by measuring urinary albumin (Mouse Albumin AssayMax ELISA Kit, Assaypro, Saint Charles, MO, USA, Cat# EMA3201-1) and urinary creatinine (Cayman Creatinine ELISA Kit, Item No. 502330, Michigan, USA), and subsequently, the urinary albumin-to-creatinine ratio (UACR) was calculated [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Control